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KMID : 1098420080160020074
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Korean Journal of Medicinal Crop Science
2008 Volume.16 No. 2 p.74 ~ p.78
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Biotransformation of Liquiritin in Glycyrrhiza uralensis Fisch Extract into Liquiritigenin by Plant Crude Enzymes
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Park Min-Ju
Min Jin-Woo
Kim Se-Young
Yang Deok-Chun
Na In-Su
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Abstract
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Liquiritin in licorice (Glycyrrhiza uralensis Fisch) extract was treated with three different plant crude enzymes (Prunus dulcis enzyme; PDE, P. armeniaca enzyme; PAE and P. persica enzyme; PPE) for biotransformation. The resulting product of liquiritin was analyzed by TLC and HPLC. The activities of crude enzymes were 259.6 U/g (PDE), 407.6 U/g (PAE) and 445.8 U/g (PPE), respectively. The liquiritin was converted to liquiritigenin after 12 hours of incubation with the crude enzymes. Liquiritigenin content reached its maximum level after the treatment with PPE at 37℃.
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KEYWORD
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Biotransformation, Liquiritin, Liquiritigenin, Plant Crude Enzymes
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